Characteristics of ISFET-type .BETA.-Glucosidic Glucoside Sensor Using Thermophilic Enzymes .BETA.-Glucosidase and Glucokinase.

Comparative analyses of two thermophilic enzymes exhibiting both beta-1,4 mannosidic and beta-1,4 glucosidic cleavage activities from Caldanaerobius polysaccharolyticus.

The hydrolysis of polysaccharides containing mannan requires endo-1,4-beta-mannanase and 1,4-beta-mannosidase activities. In the current report, the biochemical properties of two endo-beta-1,4-mannanases (Man5A and Man5B) from Caldanaerobius polysaccharolyticus were studied. Man5A is composed of an N-terminal signal peptide (SP), a catalytic domain, two carbohydrate-binding modules (CBMs), and ...

Glucokinase as pancreatic beta cell glucose sensor and diabetes gene.

Franz Matschinsky, * Yin Liang, * Prabakaran Kesavan, * Liqun Wang, * Philippe Froguel,t Gilberto Velho,t Daniel Cohen,t M. Alan Permutt,I Yukio Tanizawa,1 Thomas L. Jetton,11 Kevin Niswender,11 and Mark A. Magnuson,, *Department ofBiochemistry and Biophysics, and the Diabetes Research Center, University ofPennsylvania, Philadelphia, Pennsylvania 19104; tCentre d'Etude du Polymorphisme Humain, ...

Beta-Glucosidase From Streptomyces Griseus: Nanoparticle Immobilisation and Alkyl Glucoside Synthesis

Transglucosylation of tertiary alcohols using cassava beta-glucosidase.

We have compared the ability of beta-glucosidases from cassava, Thai rosewood, and almond to synthesize alkyl glucosides by transglucosylating alkyl alcohols of chain length C(1)-C(8). Cassava linamarase shows greater ability to transfer glucose from p-nitrophenyl-beta-glucoside to secondary alcohol acceptors than other beta-glucosidases, and is unique in being able to synthesize C(4), C(5), an...

Enhancement of Escherichia coli cellulolytic activity by co- production of beta-glucosidase and endoglucanase enzymes

Cellulase is a group of enzymes (endoglucanase, exoglucanase and betaglucosidase) required for cellulosic feedstock hydrolysis during bioethanol production. The use of recombinant cellulase is a strategy to reduce the enzyme cost. In this context, the present work describes the construction of a cellulase expression vector (pEglABglA), which allowed constitutive co-expression of endoglucanase A...